The evidence on the role of gut microbiota in post-infectious irritable bowel syndrome (PI-IBS) is convincing. Lactobacillus spp. positively aﬀects IBS symptoms, although the mechanisms through which probiotics exert their beneﬁcial eﬀects are largely unknown. The aim of the study is to evaluate the role of Lactobacillus casei DG (LC-DG) and its postbiotic (PB) in modulating the inﬂammatory/immune-response in PI-IBS in an exvivo organ culture model.
Ex vivo cultures of ileal and colonic mucosa from 10 PI-IBS, diarrhea predominant subtype (D) patients, and 10 healthy controls (HC) were treated with LPS, LCDG and PB. Interleukin (IL)-1α, IL-6, IL-8 and IL-10 mRNA levels were assessed by real-time PCR and Toll like receptor 4 (TLR-4) protein expression by Western blotting.
At baseline, IL-1α, IL-6 and IL-8 mRNA levels as well as TLR-4 protein expression were signiﬁcantly higher while IL-10 mRNA levels were lower in PI-IBS D than in HC in both ileum and colon. LC-DG and PB signiﬁcantly reduced the mRNA levels of pro-inﬂammatory cytokines and TLR-4 while increased that of IL-10 after LPS stimulation. The protective eﬀect was more pronounced for PB than LC-DG treatment.
LC-DG and its PB attenuate the inﬂammatory mucosal response in an exvivo organ culture model of PI-IBS D.
Compare D, Rocco A, Coccoli P, Angrisani D, Sgamato C, Iovine B, Salvatore U, Nardone G.
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